DNA

Part:BBa_K3878020:Design

Designed by: Louise Merle   Group: iGEM21_UParis_BME   (2021-09-27)


T7 promoter +hsa-miRNA-21_5p + T7 terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 194
    Illegal SpeI site found at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 194
    Illegal SpeI site found at 230
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 194
    Illegal SpeI site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Must be expressed in bacteria using T7 RNA polymerase. This part contains the restriction site BglII at its 5' and BamHI at its 3'.


Source

Ordered from IDT

References